Description
Features
- Standardized method for a variety of sample types
- High yields even from specialized samples
- High-quality DNA
- Optimized protocols for a range of starting materials
- Spin-column and 96-well high-throughput formats
- Automate workflows on the QIAcube Connect
Principle
DNeasy Blood & Tissue Kits are designed for rapid purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen animal tissues and cells, blood, or bacteria.
The DNeasy membrane combines the binding properties of a silica-based membrane with simple microspin technology or with the QIAGEN 96-Well-Plate Centrifugation System. DNA adsorbs to the DNeasy membrane in the presence of high concentrations of chaotropic salt, which remove water from hydrated molecules in solution. Buffer conditions in DNeasy Blood & Tissue procedures are designed to enable specific adsorption of DNA to the silica membrane and optimal removal of contaminants and enzyme inhibitors.
Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. This makes DNeasy Blood & Tissue Kits highly suited for simultaneous processing of multiple samples. For higher-throughput applications, the DNeasy 96 Blood & Tissue Kit enables simultaneous processing of 96 or 192 samples.
Applications
DNeasy Blood & Tissue Kits provide high-quality DNA, ready to use in all downstream assays, including applications in:
- Life science research
- Livestock breeding
- Pedigree genotyping
- Veterinary pathogen research
- Routine applied testing
